Reptiles have unique sensory abilities, including both adaptations for processing auditory stimuli outside of the range of human perception and reliance on vibration as a means of navigating their environment. Unlike mammals and birds, we have a relatively limited understanding of how changes in environmental stimuli – particularly those from human-made sources – may impact reptile welfare. In 2020, the Detroit Zoo’s Holden Reptile Conservation Center (HRCC) was closed to the public for renovations. With the help of accelerometers to measure vibration and sound meters to measure sound levels, we were able to monitor fluctuations in these important environmental stimuli across the construction period. We paired these data with behavioral observations to measure welfare impacts across 10 different reptile species including Arrau turtles (Podocnemis expansa), beaded lizards (Heloderma horridum), Catalina Island rattlesnakes (Crotalus catalinensis), Chinese alligators (Alligator sinensis), dwarf caimans (Paleosuchus palpebrosus), a Fly River turtle (Carettochelys insculpta), European glass lizards (Pseudopus apodus), matamata turtles (Chelus fimbriata), a Sonoran black iguana (Ctenosaura similis) and yellow-spotted Amazon River turtles (Podocnemis unifilis). We are investigating shifts in space use, behavioral diversity and frequency of species-typical behaviors as welfare indicators to understand how species respond to the sensory stimuli from the construction activity. These data will help inform how we support these animals through future construction events and provide insight into how the soundscape and vibration of the environments we create can be modified to better support or enrich the lives of reptiles in zoos and aquariums.