An avian embryo culture system for embryogenesis using an artificial vessel: possible conservation benefits in the rescue and management of endangered avian species
Publication Type: |
Journal Article |
Year of Publication: |
2005 |
Authors: |
Takaharu Kawashima, Koji Kumata, Yoshinori Tamaki, Takashi Kuwana, Koichiro Hashimoto |
Publication/Journal: |
Zoo Biology |
Publisher: |
A Wiley Company, Inc., Wiley Subscription Services |
Keywords: |
chicken, eggshell, quail, teflon membrane, whole embryo culture |
ISBN: |
1098-2361 |
Abstract:
Abstract 10.1002/zoo.20068.abs The development of artificial means to conserve some endangered avian species seems urgently needed. We devised an eggshell-less embryo culture system for embryogenesis using chicken or quail embryos as a model system. As a result, 41/56 (73%) chicken embryos and 52/62 (83%) quail embryos with their own thick albumen developed as usual for 72 and 55 hr, respectively, using a Teflon membrane (Milliwrap) and an egg-shaped plastic vessel. Furthermore, this study revealed that the most suitable vessel for culture would have the same size as the intact original eggshell. This technique opens the way for the rescue of endangered avian species even if their eggshells are abnormal or cracked. This new procedure also facilitates investigation of developmental events in many unknown birds. We discuss various aspects of the embryological techniques applicable to endangered avian species. Zoo Biol 00:000–000, 2005. © 2005 Wiley-Liss, Inc.