A comparison of sex steroid hormone excretion and metabolism by psittacine species

Publication Type:
 Journal Article
Year of Publication:
 1999
Authors:
 Julian Lee, Lisa Tell, Bill Lasley
Publication/Journal:
 Zoo Biology
Publisher:
 Inc., John Wiley & Sons
Keywords:
 , , , ,
ISBN:
 1098-2361
Abstract:

Abstract 10.1002/(SICI)1098-2361(1999)18:4<247::AID-ZOO1>3.3.CO;2-4 The metabolism, excretory rates, and excretory patterns of carbon 14 (14C) radiolabeled estradiol (E2) and testosterone (T) were studied in female budgerigars (Melopsittacus undulatus) and orange-winged Amazon parrots (Amazona amazonica). Radiolabeled E2 and T were injected intramuscularly into six budgerigars and two orange-winged Amazon parrots. Serial fecal/urine samples were collected for 168 h post-radiolabel injection. Peak radiolabeled E2 excretion was observed at 4 h post-injection, and by 24 h, 93.3 ± 6.3 and 65.9% (range, 59.1–72.7%) of the injected radiolabel was recovered in the fecal/urine matter of budgerigars and orange-winged Amazon parrots, respectively. Similarly, peak radiolabeled T excretion was observed at 4 h post-injection with 92.7± 3.6 and 66.2% (range, 57.5–75.2%) of the injected radiolabel recovered in the fecal/urine matter by 24 h in the budgerigars and orange-winged Amazon parrots, respectively. High-performance liquid chromatography (HPLC) analysis of the fecal/urine material revealed that both parrot species excreted >80% of the radiolabel in the form of complex steroid conjugates. Immunoreactive E2 and T metabolites were detected using estrone (E1) and C-21/C-19 conjugate enzyme immunoassays, respectively. Hydrolysis of the E2 metabolites and HPLC analysis of the ether extracts revealed that E2 and E1 were the major steroid moieties. Hydrolysis of the T metabolites and HPLC analysis of the ether extracts revealed two and three major unconjugated peaks for the budgerigars and the orange-winged Amazon parrots, respectively. Zoo Biol 18:247–260, 1999. © 1999 Wiley-Liss, Inc.

Links:

Back to Resources