Molecular sex diagnosis in castoridae
Publication Type: |
Journal Article |
Year of Publication: |
2002 |
Authors: |
Ralph Kühn, Gerhard Schwab, Wolfgang Schröder, Oswald Rottmann |
Publication/Journal: |
Zoo Biology |
Publisher: |
A Wiley Company, Inc., Wiley Subscription Services |
Keywords: |
beaver, castoridae, hair root cells, noninvasive sex differentiation, sry gene |
ISBN: |
1098-2361 |
Abstract:
Abstract 10.1002/zoo.10018.abs Beavers do not exhibit any external sexual trait. Therefore, a simple method was established to differentiate between males and females by verifying the male-specific SRY gene. Genomic DNA was prepared from hair root cells. Male samples displayed an electrophoretic 157-bp band derived from the SRY gene after polymerase chain reaction (PCR) amplification. This band is missing in female samples. A 375-bp fragment of the mitochondrial cytochrome b gene served as internal control and was visible in all samples. The technique can easily be used as a standard monitoring method, and is noninvasive, quick, and reliable. Zoo Biol 21:305–308, 2002. © 2002 Wiley-Liss, Inc.