Ovarian and immunological responses to alternating exogenous gonadotropin regimens in the ocelot (Leopardus pardalis) and tigrina (Leopardus tigrinus)
Publication Type: |
Journal Article |
Year of Publication: |
2005 |
Authors: |
Regina Celia Rodrigues da Paz, William Frederick Swanson, Eduardo Antunes Dias, Cristina Harumi Adania, Valquíria Hippólito Barnabe, Renato Campanarut Barnabe |
Publication/Journal: |
Zoo Biology |
Publisher: |
A Wiley Company, Inc., Wiley Subscription Services |
Keywords: |
follicle-stimulating hormone, immunoglobulins, luteinizing-hormone, wild cats |
ISBN: |
1098-2361 |
Abstract:
Abstract 10.1002/zoo.20049.abs Exogenous gonadotropins frequently are used to stimulate ovarian follicular growth and ovulation in mammalian species, including felids. However, repeated exogenous gonadotropin treatment can result in decreased ovarian responsiveness due to antibody formation. In this study, our objectives were to assess the effectiveness of alternating gonadotropin regimens on ovarian responses in ocelots and tigrinas, and investigate the humoral immune responses to these gonadotropins in each species. Females were treated four to six times with alternating equine chorionic gonadotropin (eCG)/human chorionic gonadotropin (hCG) and porcine follicle stimulating hormone (pFSH)/luteinizing hormone (pLH) regimens at 4-month intervals. With each treatment, the females were evaluated laparoscopically to assess ovarian follicular development and recover oocytes from mature follicles. Blood was collected before each treatment and at laparoscopy. Overall, the ocelots averaged more (P<0.05) follicles and corpus luteum (CL) (6.8±0.8; mean±SEM) per stimulation than the tigrinas (2.3±0.4), but the percentage of mature oocytes (mean range=54–55%) did not differ (P<0.05). Within species, both gonadotropin regimens were equally effective (P>0.05) in inducing follicular growth and oocyte maturation. The total number of ovarian structures and oocyte maturation percentages did not decrease (P<0.05) in either species with sequential stimulations. Although the percentage of blood samples containing anti-gonadotropin immunoglobulins increased (P<0.05) with sequential treatment, the presence of positive titers did not cause a decrease (P<0.05) in ovarian responsiveness. In summary, the female ocelots and tigrinas continued to respond to these alternating ovarian stimulation protocols after repeated use, despite the formation of anti-gonadotropin antibodies in some of the females. These findings suggest that the use of alternating gonadotropin regimens may permit more intensive reproductive management of these endangered cat species for conservation. Zoo Biol 00:1–14, 2005. © 2005 Wiley-Liss, Inc.