Effect of pentobarbital and isoflurane on acute stress response in rat
Publication Type: |
Journal Article |
Year of Publication: |
2015 |
Authors: |
Xue-Yan Wu, Yu-Ting Hu, Lei Guo, Jing Lu, Qiong-Bin Zhu, Er Yu, Juan-Li Wu, Li-Gen Shi, Man-Li Huang, Ai-Min Bao |
Publication/Journal: |
Physiology & Behavior |
Keywords: |
anesthesia, isoflurane, sodium-pentobarbital, stress, stress response |
ISBN: |
0031-9384 |
Abstract:
Anesthesia administration before sacrificing animals is a common practice in stress-related studies, but the effect of anesthesia on the results remains understudied. We aimed to reveal the interference of different anesthetics, i.e. intraperitoneal (i.p.) sodium-pentobarbital injection or isoflurane inhalation, with the acute stress responses in rats. Methods Rats were randomly divided into foot shock (FS) and non-stressed control groups, and further grouped according to the sacrificing procedure: direct decapitation, decapitation after i.p. sodium-pentobarbital injection, or isoflurane inhalation. There was also a non-stressed group sacrificed by decapitation following i.p. saline injection. Plasma levels of corticosterone (CORT), testosterone and estradiol, hypothalamic stress-related molecule mRNA expression of corticotropin-releasing hormone, arginine vasopressin and oxytocin, and frontal lobe stress-related molecule mRNA expression of NMDA receptor subunit NR2B, GABAA receptor and the neuronal-type nicotinic acetylcholine receptor were measured. Results