An evaluation of CHD-Specific primer sets for sex typing of birds from feathers
Publication Type: |
Journal Article |
Year of Publication: |
2008 |
Authors: |
Alan H. K. Ong, Subramaniam Vellayan |
Publication/Journal: |
Zoo Biology |
Publisher: |
A Wiley Company, Inc., Wiley Subscription Services |
Keywords: |
molecular bird sexing, naoh boiling method, zw intron regions |
ISBN: |
1098-2361 |
Abstract:
Abstract 10.1002/zoo.20163.abs The amplification of the highly conserved chromo-helicase-DNA binding region found in both the Z and W chromosome was evaluated with three sets of primers (P8/P2, 1237L/1272H and 2550F/2718R). DNA extracted from feathers through a simple boiling method was used to address its reliability in generating the sex-linked bands. All the bird samples, including the seven bird families that have not been reported previously, were successfully amplified with the primer set 2550F/2718R. The resulting polymerase chain reaction products showed clearly resolved fragments on a conventional agarose gel electrophoresis with size differences ranging from 80 to 540 bp between the two respective ZW gene copies. Although the P8/P2 primer was not as effective under the same conditions, it was able to produce well-resolved Z and W bands from bird species under the Antidea family, whereas the 2250F/2718R primer set only produced a single amplified fragment of a different size between the male and the female. Zoo Biol 27:62–69, 2008. © 2007 Wiley-Liss, Inc.