A Suite of Microsatellite Markers Optimized for Amplification of DNA From Addax (Addax nasomaculatus) Blood Preserved on FTA Cards
Publication Type: |
Journal Article |
Year of Publication: |
2012 |
Authors: |
B. C. Heim, J. A. Ivy, E. K. Latch |
Publication/Journal: |
Zoo Biology |
Keywords: |
artiodactyla, DNA storage, filter paper, fossil rim wildlife center, genetic management |
ISBN: |
1098-2361 |
Abstract:
The addax (Addax nasomaculatus) is a critically endangered antelope that is currently maintained in zoos through regional, conservation breeding programs. As for many captive species, incomplete pedigree data currently impedes the ability of addax breeding programs to confidently manage the genetics of captive populations and to select appropriate animals for reintroduction. Molecular markers are often used to improve pedigree resolution, thereby improving the long-term effectiveness of genetic management. When developing a suite of molecular markers, it is important to consider the source of DNA, as the utility of markers may vary across DNA sources. In this study, we optimized a suite of microsatellite markers for use in genotyping captive addax blood samples collected on FTA cards. We amplified 66 microsatellite loci previously described in other Artiodactyls. Sixteen markers amplified a single product in addax, but only 5 of these were found to be polymorphic in a sample of 37 addax sampled from a captive herd at Fossil Rim Wildlife Center in the US. The suite of microsatellite markers developed in this study provides a new tool for the genetic management of captive addax, and demonstrates that FTA cards can be a useful means of sample storage, provided appropriate loci are used in downstream analyses.