Mouse aversion to isoflurane versus carbon dioxide gas

Isoflurane and carbon dioxide (CO2) gas are used for rodent euthanasia. This study compared mouse aversion to isoflurane versus gradual-fill CO2 gas, and compared two methods of isoflurane delivery: vaporizer and drop. Mouse acclimation to a light–dark apparatus was used to create a light aversion test based on an unconditioned preference for dark versus light […]