Standardization and validation of a simple, sensitive, second antibody format enzyme immunoassay for growth hormone determination in mithun (Bos frontalis) plasma

Abstract 10.1002/zoo.20069.abs To facilitate research into the action of growth hormone (GH) in mithun (Bos frontalis), we standardized and validated a simple and highly sensitive enzyme immunoassay (EIA) for GH determination in mithun blood plasma on microtiter plates using biotin-streptavidin amplification system and the second antibody coating technique. Biotin was coupled to GH and used […]