Characteristics and in vitro fertilizing ability of giant panda (Ailuropoda melanoleuca) frozen-thawed epididymal spermatozoa obtained 4 hours postmortem: A case report

Abstract 10.1002/zoo.10133.abs When Chu-Lin, a male giant panda (studbook #249), died at Madrid Zoo, his reproductive tract was removed 4 hr postmortem and the epididymal spermatozoa were collected. Extended sperm were kept at 5°C for 4 hr, loaded into straws, and frozen for 7 min in liquid nitrogen vapor before the straws were plunged into […]