Extraction, PCR amplification, and sequencing of mitochondrial DNA from scent mark and feces in the giant panda

Abstract 10.1002/(SICI)1098-2361(1998)17:63.3.CO;2-G To expand the feasibility of applying simple, efficient, non-invasive DNA preparation methods using samples that can be obtained from giant pandas living in the wild, we investigated the use of scent markings and fecal samples. Giant panda–specific oligonucleotide primers were used to amplify a portion of the mitochondrial DNA control region as well […]

Captive breeding of squirrel monkeys, Saimiri sciureus and Saimiri boliviensis: The problem of hybrid groups

Abstract 10.1002/(SICI)1098-2361(1998)17:23.3.CO;2-9 The electrophoretic variability of blood proteins coding for up to 32 genetic loci was analyzed in 108 squirrel monkeys (Saimiri sciureus, Saimiri boliviensis, hybrids) from two captive colonies. Twelve polymorphic loci with 31 alleles are reported. The biallelic Ada* locus, G-statistics and Hardy-Weinberg genotype equilibria are useful for recognizing hybrids between S. sciureus […]

Genetic structure analysis of a highly inbred captive population of the African antelope Addax nasomaculatus. Conservation and management implications

Abstract The African antelope Addax nasomaculatus is a rare mammal at high risk of extinction, with no more than 300 individuals in the wild and 1,700 captive animals distributed in zoos around the world. In this work, we combine genetic data and genealogical information to assess the structure and genetic diversity of a captive population […]

Genetic rescue of an inbred captive population of the critically endangered Puerto Rican crested toad (Peltophryne lemur) by mixing lineages

The Puerto Rican crested toad (Peltophryne lemur) is currently composed of a single wild population on the south coast of Puerto Rico and two captive popu-lations founded by animals from the northern and southern coasts. The main factors contributing to its decline are habitat loss, inundation of breeding ponds during storms, and impacts of invasive […]